| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Moorella thermoacetica | CCTCGCCTGTTAACCTGCA | TTGTGCCTGGCTGGTTGA | 60.00 | 59.40 | 200 | 90 |
100.00%
|
100.00%
|
| Primer 42 | Moorella thermoacetica | CTTGATGCTGGCCATGCAG | AGCCAAAACGGACGCTGT | 59.56 | 60.20 | 274 | 90 |
100.00%
|
100.00%
|
| Primer 43 | Moorella thermoacetica | TGCTCAACTGTCCCCACTG | TTCCCTGCCCTTCCAGACT | 59.55 | 60.15 | 153 | 88 |
100.00%
|
100.00%
|
| Primer 44 | Moorella thermoacetica | AGAAGTCGAGCATGTGGCC | TGGCCTTCGCTAACTTCGG | 60.08 | 60.08 | 243 | 88 |
100.00%
|
100.00%
|
| Primer 45 | Moorella thermoacetica | GCAAATCGACGCCTTGCA | ACGGCAATGGCTTCTGCA | 59.44 | 60.28 | 273 | 88 |
100.00%
|
100.00%
|
| Primer 46 | Moorella thermoacetica | TCCCTTCCTGGTTTGGGGA | TGTCCCTAAGAGCTGGGCA | 60.07 | 60.23 | 208 | 88 |
100.00%
|
100.00%
|
| Primer 47 | Moorella thermoacetica | AAGTCGCTGGCTGGGAAA | CCACAAACCCTGTCCCCAA | 59.16 | 59.77 | 155 | 88 |
100.00%
|
100.00%
|
| Primer 48 | Moorella thermoacetica | AGCGCTTGCAGCCAGAAA | ACGCTGGACCTGTTCCTTG | 60.60 | 59.93 | 203 | 87 |
100.00%
|
100.00%
|
| Primer 49 | Moorella thermoacetica | AAGCGCATCAAAGGCACC | GAGCCAGTCGTGGTTGACA | 59.04 | 59.93 | 267 | 87 |
100.00%
|
100.00%
|
| Primer 50 | Moorella thermoacetica | CCGAGCTGGTGACCATTGT | GCGGAACCGACCAGTAACA | 60.00 | 60.01 | 187 | 87 |
100.00%
|
100.00%
|